RFLP proved to be a feasible auxiliary discrimination tool for selected Hyalomma species when access to sequencing methods is not available, as for instance during field studies. In addition, a polymerase chain reaction (PCR) based on the CO1 gene amplification of ticks has been developed for the unequivocal species identification by amplicon sequencing and specific restriction endonuclease cleavage pattern analysis. To support the results of the MALDI-TOF MS tick species identification, 41tickspecimenscomprising3to5 specimens per tick species were used to create a reference spectra database, which was evaluated using the spectra of the 44 remaining tick specimens. Moreover, MALDI TOF- MS was able to confirm morphologic findings where sequencing provided ambiguous results. The 4-legs as well as the half-idiosoma of ticks can now be applied for specimen identification using two different databases. In this study, six different Hyalomma species were tested, all of which were all clearly distinguishable by mass spectrometric analyses. MALDI-TOF MS is a reliable tool for tick identification, including specimens belonging to closely related species and hardly distinguishable using morphology. For this purpose, a reference spectral library of several European, American and African tick species was established. Within the last years, MALDI-TOF MS analysis based on tick leg proteins has been shown to be a reliable method to distinguish several tick species. The direct identification of yeasts from blood culture bottles will be possible in a routine fashion with new standardized procedures. Therefore, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and restriction fragment length polymorphism (RFLP) analysis to distinguish the most common Hyalomma species from sub-Saharan Africa ( H. However, the morphologic species discrimination can be difficult for damaged or blood-fed ticks and in case of species intercrosses. This study highlights the presence of DNA from a broad range of microorganisms in hedgehogs and their ectoparasites that may be responsible for zoonoses in Algeria.The species identification of tick vectors of Crimean-Congo hemorrhagic fever virus (CCHFV), especially Hyalomma ( H.) species, is a prerequisite to understand the eco-epidemiology of this disease and to reveal vector and virus reservoir species. A total of 1483 adult ticks were collected from. Protein identification in MALDI IMS is crucial in helping to understand the physiological role of biomolecular and cellular systems. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra. In this study, we evaluate and confirm the ability of MALDI-TOF MS, to identify different tick species collected in Cameroon, considering other lines of evidence (morphology and molecular). The DNA of potential new Bartonella and Ehrlichia species were found respectively in fleas and ticks. MALDI-TOF MS has recently been proposed as an accurate tool for arthropod identification, including ticks. Rickettsia felis and Rickettsia massiliae were detected respectively in A. Leptospira interrogans was detected in kidneys. Coxiella burnetii was detected in kidneys, spleens, A. Hedgehog organs and randomly selected arthropods were screened for microorganisms using molecular methods. Ticks and fleas are vectors for numerous human and animal pathogens. Twelve hedgehogs were infested with ectoparasites, including Archaeopsylla erinacei, Rhipicephalus sanguineus s.l. These standard protocols for MALDITOF MS arthropod identification should be complemented by additional MS spectrum quality controls, to generalize their use in monitoring arthropods of medical interest. Hedgehog kidneys, spleens and ectoparasites were collected. Le MALDI-TOF MS sera intégré au niveau des tests d’identification dans la révision actuelle de l’ISO 7218, à côté des galeries biochimiques, de l’utilisation des sondes nucléiques et des tests d’agglutination. A total of 21 Atelerix algirus corpses were picked up on roadsides and gardens. Background In the last decade, an innovative approach has emerged for arthropod identification based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This study was conducted in Bouira, a north-central region of Algeria. Abstract Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. They are potential reservoirs of various zoonotic agents.
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